DNA

Part:BBa_K3978011:Design

Designed by: Haozhen He   Group: iGEM21_HiZJU-China   (2021-10-21)


hao(hydroxyacid oxidase) gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 811
    Illegal BamHI site found at 246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 811
    Illegal PstI site found at 1552
    Illegal AgeI site found at 270
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 328
    Illegal BsaI.rc site found at 709


Design Notes

Source

it origins from Nitrosomonas europaea ATCC 19718 to enable our chassis bacteria to degrade 17α-ethynylestradiol

References